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KMID : 1123920070210030721
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Korean Journal of Oriental Physiology and Pathology
2007 Volume.21 No. 3 p.721 ~ p.727
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Inhibitory Effect of Scorpion MeOH Extract on Nitric Oxide and Cytokine Production in Lipopolysaccharide - Activated Raw 264.7 Cells
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Choi Joon-Hyuck
Lee Jong-Rok
Jee Seon-Young
Kim Sang-Chan
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Abstract
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Scorpion (SCP) has been clinically used for the treatment of endogenous wind to relieve convulsion, clearing away toxins, resolving hard masses and removing obstruction in the collaterals to relieve pain. Recent studies showed that scorpion toxins that affect the activating mechanism of sodium channels and indian black scorpion venom induced anti-proliferative and apoptogenic activity against human leukemic cell lines U937 and K562. There is lack of studies regarding the effects of SCP on the immunological activities. The present study was conducted to evaluate the effect of SCP on the regulatory effects of cytokines and nitric oxide (NO) for the immunological activities in Raw 264.7 cells. After the treatment of SCP MeOH extract dissolved in media for 1 h prior to the addition of lipopolysaccharide (LPS: 1 g/ml), cell viability was measured by MTT assay, NO production was monitored by measuring the nitrite content in culture medium. Inducible nitric oxide synthase (iNOS) was determined by immunoblot analysis, and levels of cytokine were analyzed by sandwich immunoassays. As results, SCP inhibited the production of nitrite and nitrate (0.3 and 1.0 mg/ml), iNOS and p- protein, tumor necrosis factor- (0.3 and 1.0 mg/ml), interleukin-1 (0.3 and 1.0 mg/ml) and interleukin-6 (1.0mg/ml) in Raw 264.7 cells activated with LPS. These findings suggest that SCP can produce anti-inflammatory effect, which may play a role in adjunctive therapy in Gram-negative bacterial infections.
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KEYWORD
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Scorpion, iNOS, LPS, cytokine
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